Journal: Biomedicines
Article Title: Inhibition of the Renin-Angiotensin System Improves Hemodynamic Function of the Diabetic Rat Heart by Restoring Intracellular Calcium Regulation.
doi: 10.3390/biomedicines13030757
Figure Lengend Snippet: Figure 5. Expression of key Ca2+i cycling enzymes in T2D animals: Protein abundance of RyR2, SERCA2a, PLB, and P-PLB normalized to GAPDH and expressed as a percentage of the values measured in T2D Control (n = 4 in each group) (a). Representative Western blot images (b). Statistics: Kruskal–Wallis ANOVA on ranks and Dunn’s multiple comparison test. ***: p < 0.001 vs. T2D Control values; ##: p < 0.01 vs. T2D; (Mean ± SD). Abbreviations: Ca2+i—intracellular calcium, T2D—type 2 diabetes, RyR2—Ryanodine receptor type 2, SERCA2a—Sarcoendoplasmic reticulum Ca2+-ATPase 2a, PLB—Phopholamban, P-PLB—16Ser-phosphorylated phospholamban, GAPDH—Glyceraldehyde 3-phosphate dehydrogenase.
Article Snippet: Membranes were then blocked with 5% bovine serum albumin in tris-buffered saline containing 0.1% Tween 20 (TBST, Merck Group, Darmstadt, Germany) at room temperature for 1 h and incubated with the following primary antibodies: anti- Biomedicines 2025, 13, 757 9 of 27 Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (G-9) 1:1000; (sc-365062, Santa Cruz Biotechnology, Dallas, TX, USA), RyR2 1:1000; (Ab9080; Chemicon, Merck Group, Darmstadt, Germany), anti-SERCA2a Antibody 1:500; (NB100-237, Novus, St. Louis, MO, USA), anti-phospholamban 1:500; (FL-52, sc-30142, Santa Cruz Biotechnology, Dallas, TX, USA), anti-phospho-phospholamban 1:500 (Ser16) Antibody (07-052 Millipore, Burlington, MA, USA) overnight at 4 ◦C.
Techniques: Expressing, Quantitative Proteomics, Control, Western Blot, Comparison