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anti serca2  (Novus Biologicals)


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    Structured Review

    Novus Biologicals anti serca2
    Anti Serca2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+serca2+atpase/pm41896216-54-63-65?v=Novus+Biologicals
    Average 94 stars, based on 10 article reviews
    anti serca2 - by Bioz Stars, 2026-07
    94/100 stars

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    Novus Biologicals anti serca2a antibody
    Figure 5. Expression of key Ca2+i cycling enzymes in T2D animals: Protein abundance of RyR2, <t>SERCA2a,</t> PLB, and P-PLB normalized to GAPDH and expressed as a percentage of the values measured in T2D Control (n = 4 in each group) (a). Representative Western blot images (b). Statistics: Kruskal–Wallis ANOVA on ranks and Dunn’s multiple comparison test. ***: p < 0.001 vs. T2D Control values; ##: p < 0.01 vs. T2D; (Mean ± SD). Abbreviations: Ca2+i—intracellular calcium, T2D—type 2 diabetes, RyR2—Ryanodine receptor type 2, SERCA2a—Sarcoendoplasmic reticulum Ca2+-ATPase 2a, PLB—Phopholamban, P-PLB—16Ser-phosphorylated phospholamban, GAPDH—Glyceraldehyde 3-phosphate dehydrogenase.
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    Novus Biologicals nb100 237
    Figure 5. Expression of key Ca2+i cycling enzymes in T2D animals: Protein abundance of RyR2, <t>SERCA2a,</t> PLB, and P-PLB normalized to GAPDH and expressed as a percentage of the values measured in T2D Control (n = 4 in each group) (a). Representative Western blot images (b). Statistics: Kruskal–Wallis ANOVA on ranks and Dunn’s multiple comparison test. ***: p < 0.001 vs. T2D Control values; ##: p < 0.01 vs. T2D; (Mean ± SD). Abbreviations: Ca2+i—intracellular calcium, T2D—type 2 diabetes, RyR2—Ryanodine receptor type 2, SERCA2a—Sarcoendoplasmic reticulum Ca2+-ATPase 2a, PLB—Phopholamban, P-PLB—16Ser-phosphorylated phospholamban, GAPDH—Glyceraldehyde 3-phosphate dehydrogenase.
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    Novus Biologicals anti serca2 atpase
    Figure 5. Expression of key Ca2+i cycling enzymes in T2D animals: Protein abundance of RyR2, <t>SERCA2a,</t> PLB, and P-PLB normalized to GAPDH and expressed as a percentage of the values measured in T2D Control (n = 4 in each group) (a). Representative Western blot images (b). Statistics: Kruskal–Wallis ANOVA on ranks and Dunn’s multiple comparison test. ***: p < 0.001 vs. T2D Control values; ##: p < 0.01 vs. T2D; (Mean ± SD). Abbreviations: Ca2+i—intracellular calcium, T2D—type 2 diabetes, RyR2—Ryanodine receptor type 2, SERCA2a—Sarcoendoplasmic reticulum Ca2+-ATPase 2a, PLB—Phopholamban, P-PLB—16Ser-phosphorylated phospholamban, GAPDH—Glyceraldehyde 3-phosphate dehydrogenase.
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    Novus Biologicals recombinant serca2 protein
    Fig. 1. Presence and localization of <t>SERCA2</t> in pig sperm. (a) Representative immunoblotting of SERCA2 in pig sperm after incubation with the anti-SERCA2 antibody. MW: Molecular Weight; 1–3 correspond to different samples. The black box indicates the SERCA2 band. (b) Representative immunofluorescence analysis of SERCA2 in pig sperm. Sperm nuclei are shown in blue (DAPI), whereas SERCA2 is shown in green. The white arrow shows the localization of SERCA2 in the midpiece, whereas arrowheads show its presence in the acrosome. Scale bars: 20 μm.
    Recombinant Serca2 Protein, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+serca2+atpase/pm39905176-230-13-18?v=Novus+Biologicals
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    Figure 5. Expression of key Ca2+i cycling enzymes in T2D animals: Protein abundance of RyR2, SERCA2a, PLB, and P-PLB normalized to GAPDH and expressed as a percentage of the values measured in T2D Control (n = 4 in each group) (a). Representative Western blot images (b). Statistics: Kruskal–Wallis ANOVA on ranks and Dunn’s multiple comparison test. ***: p < 0.001 vs. T2D Control values; ##: p < 0.01 vs. T2D; (Mean ± SD). Abbreviations: Ca2+i—intracellular calcium, T2D—type 2 diabetes, RyR2—Ryanodine receptor type 2, SERCA2a—Sarcoendoplasmic reticulum Ca2+-ATPase 2a, PLB—Phopholamban, P-PLB—16Ser-phosphorylated phospholamban, GAPDH—Glyceraldehyde 3-phosphate dehydrogenase.

    Journal: Biomedicines

    Article Title: Inhibition of the Renin-Angiotensin System Improves Hemodynamic Function of the Diabetic Rat Heart by Restoring Intracellular Calcium Regulation.

    doi: 10.3390/biomedicines13030757

    Figure Lengend Snippet: Figure 5. Expression of key Ca2+i cycling enzymes in T2D animals: Protein abundance of RyR2, SERCA2a, PLB, and P-PLB normalized to GAPDH and expressed as a percentage of the values measured in T2D Control (n = 4 in each group) (a). Representative Western blot images (b). Statistics: Kruskal–Wallis ANOVA on ranks and Dunn’s multiple comparison test. ***: p < 0.001 vs. T2D Control values; ##: p < 0.01 vs. T2D; (Mean ± SD). Abbreviations: Ca2+i—intracellular calcium, T2D—type 2 diabetes, RyR2—Ryanodine receptor type 2, SERCA2a—Sarcoendoplasmic reticulum Ca2+-ATPase 2a, PLB—Phopholamban, P-PLB—16Ser-phosphorylated phospholamban, GAPDH—Glyceraldehyde 3-phosphate dehydrogenase.

    Article Snippet: Membranes were then blocked with 5% bovine serum albumin in tris-buffered saline containing 0.1% Tween 20 (TBST, Merck Group, Darmstadt, Germany) at room temperature for 1 h and incubated with the following primary antibodies: anti- Biomedicines 2025, 13, 757 9 of 27 Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (G-9) 1:1000; (sc-365062, Santa Cruz Biotechnology, Dallas, TX, USA), RyR2 1:1000; (Ab9080; Chemicon, Merck Group, Darmstadt, Germany), anti-SERCA2a Antibody 1:500; (NB100-237, Novus, St. Louis, MO, USA), anti-phospholamban 1:500; (FL-52, sc-30142, Santa Cruz Biotechnology, Dallas, TX, USA), anti-phospho-phospholamban 1:500 (Ser16) Antibody (07-052 Millipore, Burlington, MA, USA) overnight at 4 ◦C.

    Techniques: Expressing, Quantitative Proteomics, Control, Western Blot, Comparison

    Fig. 1. Presence and localization of SERCA2 in pig sperm. (a) Representative immunoblotting of SERCA2 in pig sperm after incubation with the anti-SERCA2 antibody. MW: Molecular Weight; 1–3 correspond to different samples. The black box indicates the SERCA2 band. (b) Representative immunofluorescence analysis of SERCA2 in pig sperm. Sperm nuclei are shown in blue (DAPI), whereas SERCA2 is shown in green. The white arrow shows the localization of SERCA2 in the midpiece, whereas arrowheads show its presence in the acrosome. Scale bars: 20 μm.

    Journal: Scientific reports

    Article Title: The Sarcoplasmic/Endoplasmic reticulum Ca 2+ -ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation.

    doi: 10.1038/s41598-025-88012-5

    Figure Lengend Snippet: Fig. 1. Presence and localization of SERCA2 in pig sperm. (a) Representative immunoblotting of SERCA2 in pig sperm after incubation with the anti-SERCA2 antibody. MW: Molecular Weight; 1–3 correspond to different samples. The black box indicates the SERCA2 band. (b) Representative immunofluorescence analysis of SERCA2 in pig sperm. Sperm nuclei are shown in blue (DAPI), whereas SERCA2 is shown in green. The white arrow shows the localization of SERCA2 in the midpiece, whereas arrowheads show its presence in the acrosome. Scale bars: 20 μm.

    Article Snippet: To confirm the specificity of the primary antibody, a competition assay using a recombinant SERCA2 protein (ref. H00000488-P01; Novus Biologicals; 1:1, v: v, regarding the primary antibody) was run.

    Techniques: Western Blot, Incubation, Molecular Weight, Immunofluorescence